Immunorelatedness of oligopeptidases from Lactobacillus and Lactococcus and proline iminopeptidase from Propionibacterium using double immunodiffusion assay
L. Stepaniak et al., Immunorelatedness of oligopeptidases from Lactobacillus and Lactococcus and proline iminopeptidase from Propionibacterium using double immunodiffusion assay, FOOD AGR IM, 13(3), 2001, pp. 183-187
Double immunodiffusion assay showed that species of lactobacilli produce im
munologically unrelated oligopeptidases. Polyclonal antibodies (PAbs) speci
fic for intracellular 70 kDa oligopeptidase type PepO from Lactobacillus cu
rvatus reacted with intracellular protein(s) of cell-free extract (CFE) fro
m Lb. paracasei and Lb. casei but not with intracellular proteins of Lb. pl
antarum, Lb. pseudoplantarum or Lb. helveticus. PAbs specific for 70 kDa ol
igopeptidase from Lactococcus lactis did not react with proteins in CFE fro
m lactobacilli but at similar PepO activity reacted, with different affinit
y, with proteins in CFE of various lactococcal cultures. Antibodies specifi
c for intracellular proline iminopeptidase from Propionibacterium freudenre
ichii reacted with proteins in the CFE from cultures of other dairy propion
ibacteria only.