Immunorelatedness of oligopeptidases from Lactobacillus and Lactococcus and proline iminopeptidase from Propionibacterium using double immunodiffusion assay

Authors
Stepaniak, L Wroblewska, B Jedrychowski, L Sorhaug, T
Citation
L. Stepaniak et al., Immunorelatedness of oligopeptidases from Lactobacillus and Lactococcus and proline iminopeptidase from Propionibacterium using double immunodiffusion assay, FOOD AGR IM, 13(3), 2001, pp. 183-187
Citations number
14
Categorie Soggetti
Food Science/Nutrition
Journal title
FOOD AND AGRICULTURAL IMMUNOLOGY
ISSN journal
09540105 → ACNP
Volume
13
Issue
3
Year of publication
2001
Pages
183 - 187
Database
ISI
SICI code
0954-0105(200109)13:3<183:IOOFLA>2.0.ZU;2-W
Abstract
Double immunodiffusion assay showed that species of lactobacilli produce im munologically unrelated oligopeptidases. Polyclonal antibodies (PAbs) speci fic for intracellular 70 kDa oligopeptidase type PepO from Lactobacillus cu rvatus reacted with intracellular protein(s) of cell-free extract (CFE) fro m Lb. paracasei and Lb. casei but not with intracellular proteins of Lb. pl antarum, Lb. pseudoplantarum or Lb. helveticus. PAbs specific for 70 kDa ol igopeptidase from Lactococcus lactis did not react with proteins in CFE fro m lactobacilli but at similar PepO activity reacted, with different affinit y, with proteins in CFE of various lactococcal cultures. Antibodies specifi c for intracellular proline iminopeptidase from Propionibacterium freudenre ichii reacted with proteins in the CFE from cultures of other dairy propion ibacteria only.