The detection of Salmonella serovars from animal feed and raw chicken using a combined immunomagnetic separation and ELISA method

The immunomagnetic separation technique was used in conjunction with an ELI SA-based detection system to recover Salmonella enteritidis, Salmonella typ himurium and Salmonella virchow (2-2 x 10(3) cfu 25 g(-1)) from raw chicken and animal feed. The detection time was less than 27 h and included the co nventional pre-enrichment (18 h) step in buffered peptone water and an enri chment step (6 h) in glucose nutrient broth. In contrast, the conventional method using Rappaport-Vassiliades enrichment broth and selective plating o n XLD agar required 72-96 h. (C) 2001 Academic Press.