Jd. Fisch et al., The Graduated Embryo Score (GES) predicts blastocyst formation and pregnancy rate from cleavage-stage embryos, HUM REPR, 16(9), 2001, pp. 1970-1975
BACKGROUND: Embryo morphology and cleavage rates alone do not consistently
identify embryos with high implantation potential following IVF. Blastocyst
transfer has been reported to improve success rates by identifying potenti
ally superior quality embryos. Algorithms for predicting IVF outcomes based
on the presence of early developmental milestones have been proposed. Here
we introduce the Graduated Embryo Score (GES). METHODS: Nucleolar alignmen
t along the pronuclear axis, regular cleavage and degree of fragmentation a
t the first cell division, and cell number and morphology on day 3 were wei
ghted to create a possible GES of 100 for each of 1245 fertilized embryos d
erived from 109 patients aged <40 years. The GES was correlated with IVF ou
tcome. RESULTS: Of 983 embryos for extended culture, 349 (36%) developed to
blastocyst and 180 (18%) were good quality (grade I-II). When ranked by ce
ll number and morphology alone, 34% of embryos with <greater than or equal
to>7 cells and <20% fragmentation formed good quality blastocysts. Using GE
S, embryos scoring 90-100 had 64% blastocyst formation compared with 31% sc
oring 70-85 and with 11% scoring 30-65. Embryos scoring 70-100 had 44% blas
tocyst development compared with 9% scoring 0-65. Fifty-six patients (51%)
conceived on-going gestations from 294 transferred embryos. In patients wit
h at least one transferred embryo scoring <greater than or equal to>70, the
pregnancy rate was 59% compared with 34% if all embryos scored <70. The ov
erall implantation rate was 28%. Among embryos scoring 70-100, an implantat
ion rate of 39% was seen, compared with 24% among embryos scoring 0-65. CON
CLUSIONS: Predicting which cleaved embryos will form blastocysts could perm
it the high success rates associated with blastocyst transfer to be achieve
d from day 3 embryo transfer.