Role of ADP-ribosyltransferase activity of pertussis toxin in toxin-adhesin redundancy with filamentous hemagglutinin during Bordetella pertussis infection

Pertussis toxin (PT) and filamentous hemagglutinin (FHA) are two major viru lence factors of Bordetella pertussis. FRA is the main adhesin, whereas PT is a toxin with an A-B structure, in which the A protomer expresses ADP-rib osyltransferase activity and the B moiety is responsible for binding to the target cells. Here, we show redundancy of FHA and PT during infection. Whe reas PT-deficient and FHA-deficient mutants colonized the mouse respiratory tract nearly as efficiently as did the isogenic parent strain, a mutant de ficient for both factors colonized substantially less well. This was not du e to redundant functions of PT and FRA as adhesins, since in vitro studies of epithelial cells and macrophages indicated that FHA, but not PT, acts as an adhesin. An FHA-deficient B. pertussis strain producing enzymatically i nactive PT colonized as poorly as did the FHA-deficient, PT-deficient strai n, indicating that the ADP-ribosyltransferase activity of PT is required fo r redundancy with FRA. Only strains producing active PT induced a local tra nsient release of tumor necrosis factor alpha (TNF-alpha), suggesting that the pharmacological effects of PT are the basis of the redundancy with FRA, through the release of TNF-alpha. This may lead to damage of the pulmonary epithelium, allowing the bacteria to colonize even in the absence of FHA.