Cloning, nucleotide sequence, and expression of the Brucella melitensis sucB gene coding for an immunogenic dihydrolipoamide succinyltransferase homologous protein

Authors
Zygmunt, MS Diaz, MA Teixeira-Gomes, AP Cloeckaert, A
Citation
Ms. Zygmunt et al., Cloning, nucleotide sequence, and expression of the Brucella melitensis sucB gene coding for an immunogenic dihydrolipoamide succinyltransferase homologous protein, INFEC IMMUN, 69(10), 2001, pp. 6537-6540
Citations number
22
Categorie Soggetti
Immunology
Journal title
INFECTION AND IMMUNITY
ISSN journal
00199567 → ACNP
Volume
69
Issue
10
Year of publication
2001
Pages
6537 - 6540
Database
ISI
SICI code
0019-9567(200110)69:10<6537:CNSAEO>2.0.ZU;2-D
Abstract
The Brucella melitensis sucB gene encoding the dihydrolipoamide succinyltra nsferase (E2o) enzyme (previously identified as an immunogenic protein in i nfected sheep) was cloned and sequenced. The amino acid sequence predicted from the cloned gene revealed 88.8 and 51.2% identity to the dihydrolipoami de succinyltransferase SucB protein from Brucella abortus and Escherichia c oli, respectively. Sera from naturally infected sheep showed antibody react ivity against the recombinant SucB protein.