Epitope mapping of neutralizing botulinum neurotoxin A antibodies by phagedisplay

Authors
Mullaney, BP Pallavicini, MG Marks, JD
Citation
Bp. Mullaney et al., Epitope mapping of neutralizing botulinum neurotoxin A antibodies by phagedisplay, INFEC IMMUN, 69(10), 2001, pp. 6511-6514
Citations number
27
Categorie Soggetti
Immunology
Journal title
INFECTION AND IMMUNITY
ISSN journal
00199567 → ACNP
Volume
69
Issue
10
Year of publication
2001
Pages
6511 - 6514
Database
ISI
SICI code
0019-9567(200110)69:10<6511:EMONBN>2.0.ZU;2-Y
Abstract
Single-chain antibodies neutralize activity and bind nonoverlapping epitope s of botulinum A neurotoxin. Two phage display epitope libraries were const ructed from the 1.3 kb of binding domain cDNA. The minimal epitopes selecte d against the single-chain Fv-Fc antibodies correspond to conformational ep itopes with amino acid residues 1115 to 1223 (S25), 1131 to 1264 (3D12), an d 889 to 1294 (C25).