Murine models of systemic lupus erythematosus: B and T cell responses to spliceosomal ribonucleoproteins in MRL/Fas(lpr) and (NZB x NZW)F-1 lupus mice

(NZB x NZW)F-1 and MRL/Fas(Ipr) lupus mice present a similar phenotype with a spectrum of autoantibodies associated with very severe nephritis. It is thought, however, that in contrast to other lupus-prone mice such as MRL/Fa s(Ipr) mice, (NZB x NZW)F-1 mice do not generate autoantibodies to ribonucl eoproteins (RNP) Sm/RNP. In this study, we demonstrate that contrary to pre vious reports, the autoimmune response directed against Sm/RNP antigens als o occurs in NZB x NZW mice. CD4(+) T cells from unprimed 10-week-old NZB x NZW mice proliferate and secrete IL-2 in response to peptide 131-151 of the U1-70K protein, which is known to contain a T-h epitope recognized by CD4 T cells from MRL/Fas(Ipr) mice. Peptide 131-151, which was found to bind I -A(k) and I-E-k class II MHC molecules, also bound both I-A(d) and I-E-d mo lecules. This result led us to also re-evaluate longitudinally the anti-Sm/ RNP antibody response in NZB x NZW mice. We found that 25-week-old mice do produce antibodies reacting with several small nuclear and heterogeneous nu clear (hn) RNP proteins, such as SmD1, Ul-70K and hnRNP A2/B1 proteins. The fine specificity of these antibodies was studied with overlapping syntheti c peptides. The same antigenically positive and negative peptides were char acterized in MRL/Fas(Ipr) and NZB x NZW mice in the three proteins. This ne w finding can help to understand the mechanisms involved in the development of the anti-Sm/RNP antibody response and, particularly, the role played by non-MHC genes in this autoimmune response.