Y. Saeki et al., Protein kinase A increases the rate of relaxation but not the rate of tension development in skinned rat cardiac muscle, JPN J PHYSL, 51(4), 2001, pp. 427-433
To clarify the contribution of crossbridge kinetics to the contraction prof
ile of cardiac twitch during P-adrenergic stimulation, we studied the rate
of tension development and relaxation following laser flash photolysis of c
aged compounds in rat-skinned ventricular trabeculae before and after treat
ment with the catalytic subunit of protein kinase A (PKA, 0.5 U/mul, 40 min
). Tension development following nitrophenyl (NP)EGTA photolysis was fitted
with a single exponential function. The rate constant increased with an in
crease in postphotolysis steady tension, and the relation between the rate
constant and the tension was not influenced by PKA. The rate of relaxation
following diazo-2 photolysis was fitted with a double exponential function.
The rate of both initial rapid and subsequent slow relaxation was independ
ent of the extent of relaxation. PKA increased the rate of initial rapid re
laxation by about twofold, but showed no significant effect on the rate of
subsequent slow relaxation. These results suggest that in beta -receptor st
imulated rat cardiac muscle, the increased rate of tension development and
the facilitated relaxation rate during twitch can be partly explained as be
ing due to the combined effects of decreased Ca2+ affinity of troponin C an
d increased cycling rate of cross-bridges (subtractive combination for tens
ion development and additive combination for tension relaxation).