In vitro culture and its application on the cloning of guava (Psidium guajava L.)

In vitro cloning of guava was achieved on MURASHIGE and SKOOG (1962) basal medium (MS) enriched with cytokinins. Using nodal explant, which proved to be superior to the shoot tip explant in this study, the highest multiplicat ion rate (total number of nodes per explant) was induced by 0.5 mgl(-1) ben zylaminopurine (BAP) after 4 weeks. Higher concentrations (2.0 ing l(-1)) o f isopentyladenine (2iP) significantly improved shoot proliferation. Combin ations of BAP and naphthaleneacetic acid (NAA) did not induce shoot growth. No difference was noticed between the morphogenetic response of the nodal explants cultured on either MS basal media or olive medium (Om).