Assessment of the retention properties of poly(vinyl alcohol) stationary phase for lipid class profiling in liquid chromatography

Potentialities of polymerized vinyl alcohol on silica gel were assessed for class separation of simple lipids, sphingolipids, glyceroglycolipids and p hospholipids by high-performance liquid chromatography. A screening of pure solvents in binary gradient elution and a chemometric approach was used to define a rugged two segment linear gradient formed from four solvents for total lipid class separation. Triethylamine and formic acid were added in a ll mobile phase components for acidic phospholipid separation and evaporati ve light scattering response enhancement. Simple analytical procedures are described for the analysis of complex lipid materials. (C) 2001 Elsevier Sc ience B.V. All rights reserved.