Fs. Deschamps et al., Assessment of the retention properties of poly(vinyl alcohol) stationary phase for lipid class profiling in liquid chromatography, J CHROMAT A, 928(2), 2001, pp. 127-137
Potentialities of polymerized vinyl alcohol on silica gel were assessed for
class separation of simple lipids, sphingolipids, glyceroglycolipids and p
hospholipids by high-performance liquid chromatography. A screening of pure
solvents in binary gradient elution and a chemometric approach was used to
define a rugged two segment linear gradient formed from four solvents for
total lipid class separation. Triethylamine and formic acid were added in a
ll mobile phase components for acidic phospholipid separation and evaporati
ve light scattering response enhancement. Simple analytical procedures are
described for the analysis of complex lipid materials. (C) 2001 Elsevier Sc
ience B.V. All rights reserved.