High-performance liquid chromatographic separation of the enantiomers of organophosphorus pesticides on polysaccharide chiral stationary phases

High-performance liquid chromatographic separation of the individual enanti omers of 12 organophosphorus pesticides (OPs) was obtained on polysaccharid e enantioselective HPLC columns using alkane-alcohol mobile phase. The OP p esticides were crotoxyphos. dialifor, fonofos, fenamiphos, fensulfothion, i sofenphos. malathion, methamidophos, profenofos, crufomate, prothiophos and trichloronate. The enantiomers of fenamiphos, fensulfothion. profenofos an d crufomate were separated on CHIRALPAK((R)) AD((R)) the enantiomers of fen amiphos were also separated on CHIRALPAK((R)) AS((R)) the enantiomers of me thamidophos. crufomate and trichloronate were separated on CHIRALCEL((R)) O D(R): the enantiomers of crotoxyphos. dialifor, fonofos, malathion, prothio phos and trichloronate were separated on CHIRALCEL((R)) OJ((R)); and the en antiomers of isofenphos were separated on CHIRALCEL((R))OG((R)). Baseline o r partial separation of the enantiomers of six of these OP pesticides was o btained on CHIRALCEL((R)) OJ((R)) In continued method development, the sepa ration of the enantiomers of the 12 OPs was investigated more extensively o n CHIRALCEL((R)) OJ to determine whether the mobile phase composition, flow -rate and column temperature could be optimized to yield at least partial s eparation of the enantiomers. Chromatographic conditions were found that ga ve either baseline or near baseline separations of the enantiomers of the 1 2 OPs on the CHIRALCEL((R)) OJ column. (C) 2001 Elsevier Science B.V. All r ights reserved.