Sl. Zhao et al., Determination of free aspartic acid enantiomers in rat brain by capillary electrophoresis with laser-induced fluorescence detection, J CHROMAT B, 762(1), 2001, pp. 97-101
Quantification of aspartic acid enantiomers in rat brain by using a chiral
capillary electrophoresis procedure is described. Amino acids were pre-colu
mn derivatized with naphthalene-2,3-dialdehyde. Enantiomeric separation was
achieved by micellar electrokinetic chromatography in the presence of meth
anol and beta -cyclodextrin as chiral selector. The chiral separation was c
oupled with laser-induced fluorescence detection. Contents Of D- and L-aspa
rtic acids in rats at different stages of growth (from I day before birth t
o 90 days after birth) were determined. D-Aspartic acid was detected in all
the brain tissue samples tested, but at different levels. In the cerebrum
of rats I day before birth, D-aspartic acid was found to be at the highest
concentration of 81 nmol/g wet tissue. The level of D-aspartic acid in rat
brain falls rapidly after birth, while the L-aspartic acid level increases
with age, (C) 2001 Elsevier Science B.V. All rights reserved.