Citation
Ma. Tawab et al., Development of a high-performance liquid chromatographic method for the determination of 11-keto-beta-boswellic acid in human plasma, J CHROMAT B, 761(2), 2001, pp. 221-227
Citations number
11
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF CHROMATOGRAPHY B
ISSN journal
13872273
→ ACNP
Volume
761
Issue
2
Year of publication
2001
Pages
221 - 227
Database
ISI
SICI code
1387-2273(20010925)761:2<221:DOAHLC>2.0.ZU;2-Q
Abstract
A validated HPLC method for the determination of 11-keto-beta -boswellic ac
id (KBA) in human plasma was developed. The method involves the solid-phase
extraction of KBA from plasma followed by a separation with reversed-phase
HPLC. Calibration was based on external standardisation and ranged between
0.1 and 2.0 mug KBA per ml plasma. Linearity was established over the enti
re calibration range and in each case the coefficient of correlation (r(2))
was above 0.99. The recovery of KBA from plasma was 85.7%. It was further
demonstrated that the method can be applied successfully to monitor the lev
el of KBA in plasma. (C) 2001 Elsevier Science B.V. All rights reserved.