Lysozyme diffusion adjacent to the (110) crystal surface

Measurements of lysozyme diffusion within the "depletion zone" of the (1 1 0) face of lysozyme crystals are reported. The measurements were performed using the technique of microscope light scattering, capable of measuring sm all sampling areas (similar to 2 mum(2)). Measurements within such small ar eas allowed for the determination of intensity correlation functions at pre cise locations (at similar to 10 mum intervals) adjacent to the (1 1 0) cry stal face. From the intensity correlation functions, diffusion coefficients of lysozyme molecules adjacent to the (1 1 0) crystal face and in solution were determined. The diffusion coefficients were observed to decrease as t he surface of the crystal was approached, Published by Elsevier Science B.V .