Mc. Wiener et Cf. Snook, The development of membrane protein crystallization screens based upon detergent solution properties, J CRYST GR, 232(1-4), 2001, pp. 426-431
A principal hypothesis of our research is that the properties of detergent
and protein/detergent solutions, such as their phase behavior and critical
phenomena, play a significant role during membrane protein crystallization.
Therefore, the characterization of these properties, and the subsequent in
corporation of this information into crystallization experiments, will enab
le the more successful formation of well-ordered membrane protein crystals
for structure determination. We are utilizing a variety of techniques for t
he development and implementation of detergent-specific membrane protein cr
ystallization screens. Observation of dye partitioning in detergent/solute
mixtures enables the relatively rapid determination of phase boundaries, an
d this information is utilized in the design of crystallization screens tha
t are currently being tested. A commercial crystallization robot has been m
odified for use in the in situ setup of crystallization screens. Lastly, th
e characterization of critical phenomena in detergent solutions has been in
itiated. (C) 2001 Published by Elsevier Science B.V.