ACID-PHOSPHATASES OF ESOX-LUCIUS - TISSUE DISTRIBUTION AND PARTIAL CHARACTERIZATION

The presence of different acid phosphatase classes was examined in org ans and tissues of the fish Esox lucius. The enzymes were separated by Sephadex G-200 column chromatography, and characterized as regards ti ssue distribution, molecular weight, metal ion activation, inhibition, pH optima, temperature sensitivity, rate of hydrolysis and substrate specificity. High and low molecular weight acid phosphatases as well a s high and low molecular weight Zn2+-dependent acid phosphatases were present in pike organs and tissues. In addition, their biochemical pro perties and tissue distribution are very similar to that of respective bird and mammalian enzymes. (C) 1997 The Fisheries Society of the Bri tish Isles.