Phylogenetic relationships among four species of snails in the genus Batill
aria that inhabit intertidal zones of the Japanese Islands were determined
from the nucleotide sequences of part of the mitochondrial gene for cytochr
ome oxidase I. The analysis indicated that B. multiformis and B. flectosiph
onata are closely related to each other and that B. zonalis is the basal of
the four species. All snails with a shell that resembled the shell of B. f
lectosiphonata that were collected from Ariake Bay and the Goto Islands had
mitochondrial DNA that corresponded to that of either B. multiformis or B.
cumingi. Our results suggest that the morphological characteristics that h
ave been used to discriminate among species in the genus Batillaria need to
be revised.