Fv. Peale et Me. Gerritsen, Gene profiling techniques and their application in angiogenesis and vascular development, J PATHOLOGY, 195(1), 2001, pp. 7-19
Citations number
103
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
The analysis of gene expression in specific tissues and physiological proce
sses has evolved over the last 20 years from the painstaking identification
of selected genes to the relatively efficient and open-ended surveying of
potentially all genes expressed in a tissue. Current art for gene discovery
includes the use of large-scale arrays of cDNA sequences or oligonucleotid
es, and molecular 'tagging' techniques such as GeneCalling and SAGE. Common
to each of these techniques is a reliance on the increasingly comprehensiv
e databases of human and mouse EST and full-length gene sequences. Early ef
forts to characterize candidate genes were limited by their narrow scope, w
hile current efforts are confounded by the enormous volume of data returned
. Sophisticated software tools are an integral part of the analysis, helpin
g to organize information into coherent groups with temporal or functional
similarity. These techniques, in conjunction with the continued analysis of
human genetic syndromes, transgenic, and knockout mice, have driven geneti
c analysis of angiogenesis and vascular development from describing which i
ndividual genes are involved to defining the outlines of regulatory network
s. Copyright (C) 2001 John Wiley & Sons, Ltd.