Tissue microarray (TMA) technology: miniaturized pathology archives for high-throughput in situ studies

Tissue microarray (TMA) technology allows a massive acceleration of studies correlating molecular in situ findings with clinico-pathological informati on. In this technique, cylindrical tissue samples are taken from up to 1000 different archival tissue blocks and subsequently placed into one empty 'r ecipient' paraffin block. Sections from TMA blocks can be used for all diff erent types of in situ tissue analyses including immunohistochemistry and i n situ hybridization. Multiple studies have demonstrated that findings obta ined on TMAs are highly representative of their donor tissues, despite the small size of the individual specimens (diameter 0.6 mm). It is anticipated that TMAs will soon become a widely used tool for all types of tissue-base d research. The availability of TMAs containing highly characterized tissue s will enable every researcher to perform studies involving thousands of tu mours rapidly. Therefore, TMAs will lead to a significant acceleration of t he transition of basic research findings into clinical applications. Copyri ght (C) 2001 John Wiley & Sons, Ltd.