P. Wang et al., Detection of bovine herpesvirus-1 in peripheral blood mononuclear cells eight months postinfection, J VET D INV, 13(5), 2001, pp. 424-427
Peripheral blood mononuclear cells (PBMCs) from 5 calves (3 controls and 2
vaccinates) used in a bovine herpesvirus 1 (BHV-1) vaccine study with a BHV
-1 Cooper strain challenge were collected 6 months after challenge. The PBM
Cs from the control animals were positive by immunofluorescence for the BHV
-1 glycoprotein D (gD) while the vaccinates were negative. The PBMC samples
from 4 of the 5 animals were examined for BHV-1 DNA by polymerase chain re
action (PCR) and for gD immunofluorescence at 8 months after challenge. The
BHV-1 DNA and viral antigen were detected in PBMC samples at 8 months post
infection, but no virus was isolated.