Novel cis-acting replication element in the adeno-associated virus type 2 genome is involved in amplification of integrated rep-cap sequences

Authors
Nony, P Tessier, J Chadeuf, G Ward, P Giraud, A Dugast, M Linden, RM Moullier, P Salvetti, A
Citation
P. Nony et al., Novel cis-acting replication element in the adeno-associated virus type 2 genome is involved in amplification of integrated rep-cap sequences, J VIROLOGY, 75(20), 2001, pp. 9991-9994
Citations number
14
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGY
ISSN journal
0022538X → ACNP
Volume
75
Issue
20
Year of publication
2001
Pages
9991 - 9994
Database
ISI
SICI code
0022-538X(200110)75:20<9991:NCREIT>2.0.ZU;2-J
Abstract
This study identifies a region of the adeno-associated virus type 2 (AAV-2) rep gene (nucleotides 190 to 540 of wild-type AAV-2) as a cis-acting Rep-d ependent element able to promote the replication of transiently transfected plasmids. This viral element is also shown to be involved in the amplifica tion of integrated sequences in the presence of adenovirus and Rep proteins .