Two pathogenesis-related peroxidases in greengram (Vigna radiata (L.) wilczek) leaves and cultured cells induced by Macrophomina phaseolina (Tassi) Goid. and its elicitor

Authors
Ramanathan, A Vidhyasekaran, P Samiyappan, R
Citation
A. Ramanathan et al., Two pathogenesis-related peroxidases in greengram (Vigna radiata (L.) wilczek) leaves and cultured cells induced by Macrophomina phaseolina (Tassi) Goid. and its elicitor, MICROBI RES, 156(2), 2001, pp. 139-144
Citations number
40
Categorie Soggetti
Biology
Journal title
MICROBIOLOGICAL RESEARCH
ISSN journal
09445013 → ACNP
Volume
156
Issue
2
Year of publication
2001
Pages
139 - 144
Database
ISI
SICI code
0944-5013(2001)156:2<139:TPPIG(>2.0.ZU;2-3
Abstract
An elicitor has been isolated from Macrophomina phaseolina, the root rot an d leaf blight pathogen of greengram. Suspension-cultured cells of greengram were established which responded to the fungal elicitor. When greengram. l eaves were inoculated with M. phaseolina two new peroxidases appeared. Simi larly, two new peroxidases could be detected in suspension-cultured greengr am cells when treated with the fungal elicitor. These peroxidases were puri fied by column chromatography and their molecular masses were 27 and 38 kDa . The new peroxidases detected in both leaves and cultured cells appear to be similar with the same molecular weights.