ATM status confers sensitivity to arsenic cytotoxic effects

Arsenic (As), a human carcinogen, represents a worldwide health problem due to the high number of people exposed to this element in their drinking wat er. Previously our group has demonstrated that As can impair lymphocyte cel l proliferation in vitro and in vivo and can increase the level of P53 prot ein, with different responses to these effects between individuals. Recentl y it has been shown that ATM protein, responsible for the autosomal recessi ve disorder ataxia telangiectasia (AT), regulates P53. In this study the in duced response of P53 was evaluated following exposure to As in human lymph oblastoid cell lines normal (+/+), heterozygous (+/-) or homozygous (-/-) f or the mutant ATM gene. After 24 h As treatment we found a dose-dependent i nduction of P53 in normal and heterozygous cell lines, although differences between cell lines were observed. An increase in P21(WAF) protein, a main effector of P53 activation, was also observed in the same cell lines. In co ntrast, neither P53 nor P21 induction was detected in homozygous cells. The ATM (+/-) and (-/-) genotypes confer more sensitivity to As cytotoxic effe cts than the normal allelic condition. Paradoxically, ATM heterozygous cell s were more sensitive to As, leading us to propose that this might be relat ed to activation of apoptosis and removal of non-repairable cells. In contr ast, in AT cells in which ATM is absent or mutated activation of P53 and it s target genes is abrogated, allowing cells to replicate with damage in the presence of As, with cell death ensuing by a pathway different from P53.