MODIFYING DUODENAL FLOW OF AMINO-ACIDS BY MANIPULATION OF DIETARY-PROTEIN SOURCES

Four multiparous Holstein dairy cows in mid-lactation were utilized in a 4 x 4 Latin square design experiment to measure the response in int estinal amino acid profiles to progressive substitution of blood meal for corn gluten meal as the protein supplement. In addition, the influ ence of these protein sources on rumen fermentation and digestion as w ell as forestomach bacterial growth and escape were compared with a di et supplemented with soybean meal, a rapidly rumen-degraded protein so urce. Cows were offered a mixed silage ration of alfalfa silage (79.8% of DM) and corn silage (20.2% of DM) twice daily. Cows were also offe red mixed concentrate at 123% (DM:DM) of the mixed silage ration in si x equal meals per day to provide soybean meal, corn gluten meal, blood meal or a combination of the latter two sources as the primary supple mental protein source. Rumen soluble non-ammonia N concentrations were higher in cows fed soybean meal and declined linearly as blood meal s ubstituted for corn gluten meal in the concentrate. Forestomach disapp earance of N tended (P = 0.09) to be higher for the cows fed soybean m eal vs. all other diets, and tended (P = 0.09) to be higher with the c ombined corn gluten meal and blood meal diet vs. the diets containing either alone. The latter is consistent with higher forestomach digesti on of DM and OM in cows fed the combined diet. Rumen pool sizes of mos t DM components were lower when cows were fed soybean meal and, with t he exception of N pools, increased linearly as blood meal substituted for corn gluten meal. Duodenal flow of amino acid protein was lower wh en cows were fed soybean meal, due mainly to reduced bacterial flow, a nd was lowest for the combined diet within the corn gluten meal and bl ood meal diets. The amino acid profile of duodenal protein differed fo r 12 of 17 amino acids examined when cows were fed the soybean meal di et vs. the other diets, and 12 of 17 amino acids either increased or d ecreased linearly as blood meal substituted for corn gluten meal. Data reported here and by previous researchers suggest an associative effe ct of addition of com gluten meal and blood meal to the diet which inc reased rumen degradation of dietary protein. These data also show that manipulating the dietary source of supplemental protein can influence the amino acid profile of duodenally delivered amino acid protein as well as indicate that the rate of rumen degradation of individual amin o acids differs among protein sources. In this study, methionine, cyst ine and histidine appeared to be more rapidly degraded in corn gluten meal than in blood meal, whereas glutamic acid, proline, isoleucine, t hreonine and lysine were more rapidly degraded in blood meal.