Immunoneutralization of somatostatin, insulin, and glucagon causes alterations in islet cell secretion in the isolated perfused human pancreas

Citation
Fc. Brunicardi et al., Immunoneutralization of somatostatin, insulin, and glucagon causes alterations in islet cell secretion in the isolated perfused human pancreas, PANCREAS, 23(3), 2001, pp. 302-308
Citations number
34
Categorie Soggetti
da verificare
Journal title
PANCREAS
ISSN journal
08853177 → ACNP
Volume
23
Issue
3
Year of publication
2001
Pages
302 - 308
Database
ISI
SICI code
0885-3177(200110)23:3<302:IOSIAG>2.0.ZU;2-N
Abstract
Introduction: In this study, immunoneutralization of endogenous insulin, gl ucagon, and somatostatin with specific antibodies was used in an isolated p erfused human pancreas (IPHP) model. Aims: To study intrapancreatic cellular interactions and pancreatic hormona l secretion. Methodology: Randomized, sequential 10-minute test intervals of single-pass per-fusion with each antibody were performed at 3.9 mM or 11.5 mM steady-s tate glucose concentrations. Somatostatin, insulin, and glucagon levels wer e measured in the effluent during basal and immunoneutralization intervals. Results: At 3.9 mM glucose concentration, somatostatin antibody (SS-Ab) sti mulated insulin and glucagon secretion, insulin antibody (IN-Ab) inhibited glucagon secretion, and glucagon antibody (GN-Ab) stimulated insulin secret ion. At 11.5 mM glucose concentration, SS-Ab stimulated insulin secretion, IN-Ab stimulated glucagon and inhibited somatostatin secretion, and GN-Ab s timulated insulin secretion. Conclusion: The variation in hormonal responses to immunoneutralization dur ing stimulated and nonstimulated glucose conditions suggests that a dynamic association exists between the pancreatic cells.