Fc. Brunicardi et al., Immunoneutralization of somatostatin, insulin, and glucagon causes alterations in islet cell secretion in the isolated perfused human pancreas, PANCREAS, 23(3), 2001, pp. 302-308
Introduction: In this study, immunoneutralization of endogenous insulin, gl
ucagon, and somatostatin with specific antibodies was used in an isolated p
erfused human pancreas (IPHP) model.
Aims: To study intrapancreatic cellular interactions and pancreatic hormona
l secretion.
Methodology: Randomized, sequential 10-minute test intervals of single-pass
per-fusion with each antibody were performed at 3.9 mM or 11.5 mM steady-s
tate glucose concentrations. Somatostatin, insulin, and glucagon levels wer
e measured in the effluent during basal and immunoneutralization intervals.
Results: At 3.9 mM glucose concentration, somatostatin antibody (SS-Ab) sti
mulated insulin and glucagon secretion, insulin antibody (IN-Ab) inhibited
glucagon secretion, and glucagon antibody (GN-Ab) stimulated insulin secret
ion. At 11.5 mM glucose concentration, SS-Ab stimulated insulin secretion,
IN-Ab stimulated glucagon and inhibited somatostatin secretion, and GN-Ab s
timulated insulin secretion.
Conclusion: The variation in hormonal responses to immunoneutralization dur
ing stimulated and nonstimulated glucose conditions suggests that a dynamic
association exists between the pancreatic cells.