Anti body-dependent cell-mediated cytotoxicity to newly excysted juvenile Fasciola hepatica in vitro is mediated by reactive nitrogen intermediates

Citation
D. Piedrafita et al., Anti body-dependent cell-mediated cytotoxicity to newly excysted juvenile Fasciola hepatica in vitro is mediated by reactive nitrogen intermediates, PARASITE IM, 23(9), 2001, pp. 473-482
Citations number
40
Categorie Soggetti
Immunology
Journal title
PARASITE IMMUNOLOGY
ISSN journal
01419838 → ACNP
Volume
23
Issue
9
Year of publication
2001
Pages
473 - 482
Database
ISI
SICI code
0141-9838(200109)23:9<473:ABCCTN>2.0.ZU;2-W
Abstract
Passive intraperitoneal transfer of sera from Fasciola hepatica-infected sh eep, cattle or rats can protect naive rats from F. hepatica infection, sugg esting a parasite killing mechanism within the peritoneal cavity that is de pendent on the presence of parasite-specific antibody. We investigated anti body-dependent cell-mediated cytotoxicity by resident peritoneal lavage cel l populations, containing large numbers of monocytes/macrophages, as a pote ntial host resistance mechanism by which juvenile flukes could be killed wi thin the peritoneal cavity of naive rats. Comparative studies were conducte d using cell populations containing large numbers of monocytes/macrophages from sheep. The results demonstrate that monocyte/macrophage-rich lavage ce ll populations from rat and sheep differ substantially in their ability to generate nitric oxide. Only resident rat peritoneal lavage cells were able to mediate antibody-dependent cell-mediated cytotoxicity against newly excy sted juvenile liver fluke. The mechanism of cytotoxicity was dependent on, and directly proportional to, the production of nitric oxide and required a ttachment of effector cells to the newly excysted juvenile liver fluke tegu ment, which occurred following the addition of sera from F. hepatica-infect ed animals. This is the first report demonstrating a mechanism of cell-medi ated cytotoxicity to newly excysted juvenile liver fluke.