CALIBRATION OF MICROPIPETS USING THE BIOLUMINESCENT PROTEIN AEQUORIN

Authors
GROSVENOR AL CROFCHECK CL ANDERSON KW SCOTT DL DAUNERT S
Citation
Al. Grosvenor et al., CALIBRATION OF MICROPIPETS USING THE BIOLUMINESCENT PROTEIN AEQUORIN, Analytical chemistry, 69(15), 1997, pp. 3115-3118
Citations number
13
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical chemistry → ACNP
ISSN journal
00032700
Volume
69
Issue
15
Year of publication
1997
Pages
3115 - 3118
Database
ISI
SICI code
0003-2700(1997)69:15<3115:COMUTB>2.0.ZU;2-Y
Abstract
A new method for calibrating micropipets and determining accurate inje ction volumes using a pressure-based injector has been developed. This method employs the bioluminescent protein aequorin and can be used to determine injection volumes as small as 3 pt. The calibration plots a re linear over at least 3 orders of magnitude. In contrast to conventi onal micropipet calibration methods that employ fluorescent molecules, the present method produces small background signals.