This study documents latent infection of Austrian and Scots pine tissues by
Sphaeropsis sapinea, the causal agent of Sphaeropsis tip blight disease. S
ymptomless shoots from diseased or apparently healthy Austrian and Scots pi
ne trees were cultured and analyzed for the presence of S. sapinea. Nearly
half of the sampled Austrian and Scots pine trees that appeared to be compl
etely healthy had detectable latent S. sapinea infections. More than half o
f the symptomless shoots on visibly diseased Austrian and Scots pine trees
were also latently infected with S. sapinea. S. sapinea was isolated from s
ymptomless shoot stems, needles, buds, immature cones, and male flowers. In
symptomless infected shoots, the fungus was primarily associated with the
bark and phloem tissues. Six isolates of S. sapinea from symptomless Austri
an and Scots pine shoot tissues were pathogenic on Austrian pine seedlings
in the greenhouse. The presence of S. sapinea in symptomless tissues of tip
blight-diseased trees has important implications for disease management.