The chromosomes 1, Y-1, Y-2 of Muntjac munticus vaginalis were isolated by
fluorescence activated chromosome sorting and amplified by degenerate oligo
nucleotide primed-polymerase chain reaction ( DOP-PCR). A primer pair withi
n human Sry HMG box was designed and the Sry gene of the male M. m vaginali
s was amplified. The product was cloned and sequenced. The result proved th
at Sry is located on chromosome Y-2, which is the sex-determining chromosom
e in the male M. m vaginalis.