H. Bazin et al., Homogeneous time resolved fluorescence resonance energy transfer using rare earth cryptates as a tool for probing molecular interactions in biology, SPECT ACT A, 57(11), 2001, pp. 2197-2211
Citations number
90
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
A homogeneous assay technology using time resolved fluorescence and fluores
cence resonance energy transfer is described. A new class of fluorescent co
mplexes, the cryptates, have been used as fluorescent donor with cross-link
ed allophycocyanin as acceptor. This new donor/acceptor shows an exceptiona
lly high Forster distance R-0 of 9 nm. This allows to build up a set of str
ategies to probe the interactions of biomolecules in biology, particularly
for high throughput screening applications. In this article, we describe th
e basics of the technology and review applications developed for studying d
ifferent key molecular interactions involved in cellular processes. (C) 200
1 Elsevier Science B.V. All rights reserved.