Recent work on the emerging application of multiphoton excitation to fluore
scence studies of biomolecular dynamics. and structure is reviewed, The fun
damental principles and experimental techniques of multiphoton excitation a
re outlined, fluorescence lifetimes, anisotropy and spectra in membranes, p
roteins, hydrocarbons, skin, tissue and metabolites are featured, and futur
e opportunities are highlighted. (C) 2001 Elsevier Science B.V. All rights
reserved.