A truncated form of teratocarcinoma-derived growth factor-1 (cripto-1) mRNA expressed in human colon carcinoma cell lines and tumors

Citation
G. Baldassarre et al., A truncated form of teratocarcinoma-derived growth factor-1 (cripto-1) mRNA expressed in human colon carcinoma cell lines and tumors, TUMOR BIOL, 22(5), 2001, pp. 286-293
Citations number
34
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
TUMOR BIOLOGY
ISSN journal
10104283 → ACNP
Volume
22
Issue
5
Year of publication
2001
Pages
286 - 293
Database
ISI
SICI code
1010-4283(200109/10)22:5<286:ATFOTG>2.0.ZU;2-8
Abstract
The human teratocarcinoma-derived growth factor (TDGF)-1 gene encodes a 188 -amino acid protein, cripto-1. The TDGF-1 gene is overexpressed in the majo rity of human primary colorectal carcinomas and hepatic metastases, in brea st carcinomas and in testicular nonseminoma germ cell embryonal carcinomas. In the human embryonal carcinoma cell line NTERA-2 clone D1, a 2-kb TDGF-1 mRNA transcript is expressed. The present study shows that a 1.7-kb mRNA t ranscript lacking the first two exons of the TDGF-1 gene is expressed in th e human colon carcinoma cell line GEO. This shorter mRNA is the only TDGF-1 transcript that is present in the majority of primary human colorectal car cinomas and hepatic metastases and in adult human tissues such as the pancr eas, heart, stomach, mammary gland, skeletal muscle, liver and placenta. In contrast, in the kidney, brain, testis, ovary and spleen, the longer 2-kb TDGF-1 mRNA transcript is expressed. The putative shorter protein starts at a CUG codon 129 nucleotides downstream of the starting AUG codon of the lo nger protein. These data indicate the potential for differential transcript ional regulation of the TDGF-1 gene in different normal and tumor tissues. Copyright (C) 2001 S. Karger AG, Basel.