Nonneutralizing human antibody fragments against hepatitis C virus E2 glycoprotein modulate neutralization of binding activity of human recombinant Fabs
R. Burioni et al., Nonneutralizing human antibody fragments against hepatitis C virus E2 glycoprotein modulate neutralization of binding activity of human recombinant Fabs, VIROLOGY, 288(1), 2001, pp. 29-35
Evidence from clinical and experimental studies indicates that hepatitis C
virus E2 (HCV/E2) glycoprotein is the major target of a putatively protecti
ve immune response. However, even in the presence of a vigorous production
of anti-HCV/E2 antibodies, reinfection can occur. Dissection of the human i
mmune response against HCV/E2 indicated that blocking of binding of HCV/E2
to target cells [neutralization of binding (NOB) activity] varies widely am
ong antibody clones. Moreover, in vivo, simultaneous binding of antibodies
to distinct epitopes can induce conformational changes and synergies that m
ay be relevant to understanding the anti-HCV immune response. In this study
, human recombinant Fabs were generated by affinity-selecting a phage displ
ay repertoire library with antibody-coated HCV/E2. These Fabs, which share
the same complementarity-determining region DNA sequences, had higher affin
ity than other anti-HCV/E2 Fabs but showed no NOB activity even at the high
est concentrations, Binding of Fabs to HCV/E2 caused conformational changes
modifying Fab-binding patterns and reducing, with a negative synergistic e
ffect, Fab-mediated NOB activity. These data suggest that some antibody clo
nes have the potential to modify HCV/E2 conformation and that in this state
, binding of this glycoprotein to its cellular target is less prone to inhi
bition by some antibody clones. This can explain why high anti-HCV/E2 antib
ody titers do not directly correlate with protection from infection. Inform
ation on the interactions among different antibody clones can contribute to
understanding virus-host interplay and developing more effective vaccines.
(C) 2001 Academic Press.