We report on the first Puumala hantavirus nucleotide sequence (strain Opina
-916) amplified from a bank vole trapped in Slovakia, central Europe. Phylo
genetic analysis of the S-segment sequence grouped the virus within the wes
tern/central European sublineage of Puumala virus. In the neighborhood of t
he rodent trapping site two cases of human infection by the Puumala virus w
ere verified.