Direct in vivo transfer of plasmid DNA into murine tumors: Effects of endotoxin presence and transgene localization

Authors
Budryk, M Cichon, T Szala, S
Citation
M. Budryk et al., Direct in vivo transfer of plasmid DNA into murine tumors: Effects of endotoxin presence and transgene localization, ACT BIOCH P, 48(3), 2001, pp. 795-800
Citations number
21
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ACTA BIOCHIMICA POLONICA
ISSN journal
0001527X → ACNP
Volume
48
Issue
3
Year of publication
2001
Pages
795 - 800
Database
ISI
SICI code
0001-527X(2001)48:3<795:DIVTOP>2.0.ZU;2-A
Abstract
The purpose of this study was to investigate the effect of endotoxin presen ce in plasmid DNA preparations on the efficiency of transfection achieved i n vivo with B16(F10) and Renca tumors and to determine transgene localizati on. Our data show that endotoxin markedly decreases the efficiency of trans fection. Furthermore, the transgene transferred in vivo can be found in bot h neoplastic and normal (most likely myofibroblast) cells lying in proximit y of the administration site.