Importance of extracellular Ca2+ and intracellular Ca2+ release in ethanol-induced contraction of cerebral arterial smooth muscle

Citation
Zw. Yang et al., Importance of extracellular Ca2+ and intracellular Ca2+ release in ethanol-induced contraction of cerebral arterial smooth muscle, ALCOHOL, 24(3), 2001, pp. 145-153
Citations number
38
Categorie Soggetti
Neurosciences & Behavoir
Journal title
ALCOHOL
ISSN journal
07418329 → ACNP
Volume
24
Issue
3
Year of publication
2001
Pages
145 - 153
Database
ISI
SICI code
0741-8329(200107)24:3<145:IOECAI>2.0.ZU;2-U
Abstract
The present study was designed to investigate the roles of extracellular Ca 2+ ([Ca2+](0)) influx and intracellular free Ca2+ ([Ca2+](i)) release in et hanol-induced contractions of isolated canine cerebral arteries and primary cultured, cerebral vascular smooth muscle cells. Ethanol (20-200 mM) produ ced significant contractions in isolated canine basilar arterial rings in a concentration-dependent manner. Removal of [Ca2+](0) and pretreatment of c anine basilar arterial rings with verapamil (an antagonist of voltage-gated Ca2+ channels), thapsigargin (a selective antagonist of the sarcoplasmic r eticulum Ca2+ pump), caffeine plus ryanodine (a specific antagonist of ryan odine-sensitive Ca2+ release), or heparin (an inositol 1,4,5,-trisphosphate [InsP(3)]-mediated Ca2+ release antagonist) markedly attenuated (-50%-80%) ethanol-induced contractions. The absence of [Ca2+](0) and preincubation o f primary single smooth muscle cells obtained from canine basilar arteries with verapamil, thapsigargin, heparin, or caffeine plus ryanodine markedly attenuated (-50%-80%) the transient and sustained elevations in [Ca2+](i) i nduced by ethanol. Results of the present study suggest to us that both Ca2 + influx through voltage-gated Ca2+ channels and Ca2+ release from intracel lular stores (both InsP(3) sensitive and ryanodine sensitive) are required for ethanol-induced contractions of isolated canine basilar arteries. (C) 2 001 Elsevier Science Inc. All rights reserved.