Impaired immunoglobulin M production by incubation of hybridoma cells withethanol

Citation
E. Muhlbauer et al., Impaired immunoglobulin M production by incubation of hybridoma cells withethanol, ALCOHOL, 24(3), 2001, pp. 179-187
Citations number
43
Categorie Soggetti
Neurosciences & Behavoir
Journal title
ALCOHOL
ISSN journal
07418329 → ACNP
Volume
24
Issue
3
Year of publication
2001
Pages
179 - 187
Database
ISI
SICI code
0741-8329(200107)24:3<179:IIMPBI>2.0.ZU;2-O
Abstract
Several reports have presented results that demonstrate suppression of the immune system by ethanol. Using a hybridoma cell model, we studied the effe cts of ethanol on cell proliferation and on the production of immunoglobuli n M (IgM) antibodies. The number of cells decreased while incubated with as little as 25 mM ethanol but not in a clonal subline incapable of IgM produ ction, indicative of an increased vulnerability associated with the antibod y-producing machinery. Levels of antibodies in cell culture supernatants we re monitored by mu -heavy-chain-specific and K-light-chain-specific enzyme- linked immunosorbent assays, We found a significant decrease in antibody co ncentration at 200 mM ethanol compared with findings for nonexposed cells. In addition, lower K-chain compared with mu -chain values were monitored at ethanol concentrations of 50 mM and higher. This difference suggests irreg ular composition of the antibodies in the supernatant. Determination of IgM levels within the hybridoma cells revealed a linear increase in antibody c oncentrations by as much as three times the control levels with increasing ethanol concentrations when correlated with cell numbers, Analysis of the m RNA levels of two ethanol-inducible stress proteins, the 78-kilodalton gluc ose-regulated protein (GRP78) and the 70-kilodalton heat-shock protein (HSC 70), by quantitative Northern hybridization yielded increased mRNA in a non linear fashion. The results demonstrate that ethanol impairs IgM compositio n, whereas antibody production within hybridoma cells is increased and the assembling machinery is activated, indicating compensating processes. (C) 2 001 Elsevier Science Inc. All rights reserved.