A modified spray reagent for the detection of amino acids on thin layer chromatography plates

Authors
Laskar, S Sinhababu, A Hazra, KM
Citation
S. Laskar et al., A modified spray reagent for the detection of amino acids on thin layer chromatography plates, AMINO ACIDS, 21(2), 2001, pp. 201-204
Citations number
14
Categorie Soggetti
Biochemistry & Biophysics
Journal title
AMINO ACIDS
ISSN journal
09394451 → ACNP
Volume
21
Issue
2
Year of publication
2001
Pages
201 - 204
Database
ISI
SICI code
0939-4451(2001)21:2<201:AMSRFT>2.0.ZU;2-5
Abstract
Identification of amino acids is extremely important for the evaluation of protein structure. Thin layer chromatography is an important tool for detec ting amino acids by variety of spray reagents. Among these ninhydrin is the most popular due to its high sensitivity. However, ninhydrin produces the same purple/violet color with most amino acids. A spray reagent with high s ensitivity for easy and rapid identification of amino acids on thin-layer p lates has been introduced.