Two nuclear genes confirm mitochondrial evidence of cryptic species withinLiriomyza huidobrensis (Diptera : Agromyzidae)

Phylogenetic analysis using DNA sequence data from two nuclear genes was un dertaken to investigate phylogeographic structure within the widespread lea fmining pest Liriomyza huidobrensis (Blanchard). Parsimony analysis of 171 bp from beta -tubulin (including an intron) and 921 bp from elongation fact or-1 alpha confirms previous findings from mitochondrial sequence data of d eep phylogeographic structure indicative of cryptic species within L. huido brensis. We resurrect the name L. langei Frick for the North American crypt ic species and restrict the name L. huidobrensis to the South and Central A merican cryptic species. Results from nuclear genes also confirm previous r esults suggesting that recent invasions of this leafminer in many areas of the world are due to the spread of L. huidobrensis from South or Central Am erica.