Sj. Scheffer et Ml. Lewis, Two nuclear genes confirm mitochondrial evidence of cryptic species withinLiriomyza huidobrensis (Diptera : Agromyzidae), ANN ENT S A, 94(5), 2001, pp. 648-653
Phylogenetic analysis using DNA sequence data from two nuclear genes was un
dertaken to investigate phylogeographic structure within the widespread lea
fmining pest Liriomyza huidobrensis (Blanchard). Parsimony analysis of 171
bp from beta -tubulin (including an intron) and 921 bp from elongation fact
or-1 alpha confirms previous findings from mitochondrial sequence data of d
eep phylogeographic structure indicative of cryptic species within L. huido
brensis. We resurrect the name L. langei Frick for the North American crypt
ic species and restrict the name L. huidobrensis to the South and Central A
merican cryptic species. Results from nuclear genes also confirm previous r
esults suggesting that recent invasions of this leafminer in many areas of
the world are due to the spread of L. huidobrensis from South or Central Am
erica.