New supports for enzyme immobilization based on copolymers of vinylene carbonate and acrylamide

In this study, a series of beadlike and hydrophilic supports containing rea ctive cyclic carbonate groups for enzyme immobilization were prepared via r everse-phase suspension copolymerization of the aqueous solutions of vinyle ne carbonate (VCA), acrylamide (AA), and N,N'-methylene bis-acrylamide in p araffin oil. The supports were used as a matrix for immobilization of tryps in and showed a considerable capacity to couple with trypsin and reasonable retention of activity for the immobilized trypsin, depending on the immobi lization conditions, such as the content of VCA structural units, reaction time, and pH of the medium.