Kd. Cole et al., Controlling electrophoretic trapping of circular DNA by addition of starchpreparations to agarose gels, APPL BIOC B, 95(1), 2001, pp. 31-43
Starch preparations were added to agarose gels to enhance the electrophoret
ic trapping of circular plasmid DNA. The critical voltages required to trap
the open circular (OC) and the supercoiled (SC) forms of a 13.1-kbp plasmi
d were measured in gels composed of agarose and added starch preparations.
Modified starch preparations reduced the critical voltage required to trap
the OC form of the plasmid to approximately one-third of the control value
(in 1% agarose gels). Amylose (a fraction of starch with a low amount of br
anching) also reduced the critical voltage to trap the OC form in a similar
manner. The critical voltage to trap the SC form of the plasmid was not si
gnificantly reduced by the starch preparations. The capacity to trap OC DNA
was increased by the addition of higher amounts of the starch preparations
added to the gels. Field inversion gel electrophoresis was used to charact
erize the length of the traps in the gels. The starch preparations and amyl
ose increased the trap lengths approximately twofold. The increased trap le
ngth correlated with the decreased critical voltage required to trap the OC
form of the 13.1-kbp plasmid.