Degradation of 1,3-dichloropropene by a soil bacterial consortium and Rhodococcus sp AS2C isolated from the consortium

A bacterial consortium capable of degrading the fumigant 1,3-D ((Z)- and (E )-1,3-dichloropropene) was enriched from an enhanced soil. This mixed cultu re degraded (Z)- and (E)-1,3-D only in the presence of a suitable biodegrad able organic substrate, such as tryptone, tryptophan, or alanine. After 8 m onths of subculturing at 2- to 3-week intervals, a strain of Rhodococcus sp . (AS2C) that was capable of degrading 1,3-D cometabolically in the presenc e of a suitable second substrate was isolated. (Z)-3-chloroallyl alcohol (3 -CAA) and (Z)-3-chloroacrylic acid (3-CAAC), and (E)-3-CAA and (E)-3-CAAC w ere the metabolites of (Z)- and (E)-1,3-D, respectively. (E)-1,3-D was degr aded faster than (Z)-1,3-D by the strain AS2C and the consortium. AS2C also degraded (E)-3-CAA faster than (Z)-3-CAA. Isomerization of (E)-1,3-D to (Z )-1,3-D or the (Z) form to the (E) form did not occur.