An electrochemical multienzymatic biosensor for determination of cholesterol

This paper describes an electrochemical biosensor for free cholesterol moni toring. The sensor is a multienzymatic electrodic system in which horseradi sh peroxidase and cholesterol oxidase are simultaneously immobilized within a polymeric film. on the surface of a pyrolitic graphite electrode. From v oltammetric and amperometric (flow-injection) data obtained, the efficiency , reproducibility and stability of the system are discussed. Results obtain ed, of interest for basic and applied biochemistry, represent a first step for construction of a mediator-free biosensor with potentialities for a suc cessful application in the biosensor area. (C) 2001 Elsevier Science BN. Al l rights reserved.