THE APPLICATION OF SYNERGISTS FOR STUDYIN G HE ACTIVITY OF SOME ENZYME-SYSTEMS AND THEIR INVOLVEMENT IN INSECTICIDE ACTION

An indirect toxicology method was used to assess the monooxygenase (MO ) and nonspecific esterase (NE) activities in three synanthropic cockr oach species and four races of houseflies with the help of common and novel organophosphorus and pyrethroid synergists: piperonyl butoxide ( PB), S,S,S-tributyltrithiophosphate (TBTP), Kitazin-P, Karesan, butylm ercapto-2-thio-1,3,2-oxazaphosphorinan (AO-6), and 2-phenylmercapto-2- thio-1 ,3,2-oxazaphosphorinan (AO-2), The relationship between the coe fficient of synergistic effect (CSE) and the concentrations of MO and NE inhibitors was described, and the minimum effective concentrations with the highest synergistic effect were determined. These concentrati ons are advisable for use in the insect enzyme assays and studies of i nsecticide detoxification and insect resistance to them.