Synthesis and distribution of glycosaminoglycans in human leukemic B- and T-cells and monocytes studied using specific enzymic treatments and high-performance liquid chromatography

Identification of glycosaminoglycans (GAGs) synthesized by three human leuk aemic cell lines-Jurkat (T-cell leukaemia), Daudi (Burkitt's lymphoma, B-ce ll leukaemia) and THP-1 (acute monocytic leukemia)-and normal peripheral bl ood mononuclear cells (PBMC) and their distribution among cell membrane and culture medium were studied. GAGs were isolated using ion-exchange chromat ography on DEAE-Sephacel and their composition and fine chemical structure were studied using highperformance liquid chromatography with radiochemical detection. All cell lines synthesize chondroitin sulphate (CS) and heparan sulphate (HS) in both cell membrane and culture medium. No hyaluronan was detected using treatment with specific lyases and highly sensitive HPLC met hodology. CS is the major secreted GAG in all cell lines tested and the maj or cell retained GAG in Jurkat and Daudi. HS is the major GAG in the cell m embrane of THP-1. The amounts of distinct GAGs synthesized by all cancer ce ll lines differ from those produced by normal PBML indicating a major role of GAGs in malignant transformation of human lymphocytes and monocytes. Cop yright (C) 2001 John Wiley & Sons, Ltd.