Microbial transformation of 18 beta-glycyrrhetinic acid by Sphingomonas paucimobilis strain G5

Authors
Yoshida, K Furihata, K Habe, H Yamane, H Omori, T
Citation
K. Yoshida et al., Microbial transformation of 18 beta-glycyrrhetinic acid by Sphingomonas paucimobilis strain G5, BIOTECH LET, 23(19), 2001, pp. 1619-1624
Citations number
11
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
19
Year of publication
2001
Pages
1619 - 1624
Database
ISI
SICI code
0141-5492(200110)23:19<1619:MTO1BA>2.0.ZU;2-N
Abstract
The effects of the oxygenase inhibitors, 1-aminobenzotriazole (ABT), ketoco nazole, metyrapone and proadifen, on the metabolism of 18 beta -glycyrrheti nic acid (18 beta -GRA) in Sphingomonas paucimobilis strain G5 were investi gated. Strain G5 transformed 18 beta -GRA into a major new metabolite (M-D) in the presence of 1 mM ABT or metyrapone. M-D was purified and identified as 3 beta -hydroxy-11-oxo-olean-12-en-24,30-dioic acid by NMR and MS. Base d on the structure of M-D, we propose the metabolic pathway of 18 beta -GRA in strain G5.