The J-domain proteins of Arabidopsis thaliana: an unexpectedly large and diverse family of chaperones

Authors
Citation
Ja. Miernyk, The J-domain proteins of Arabidopsis thaliana: an unexpectedly large and diverse family of chaperones, CELL STR CH, 6(3), 2001, pp. 209-218
Citations number
53
Categorie Soggetti
Cell & Developmental Biology
Journal title
CELL STRESS & CHAPERONES
ISSN journal
13558145 → ACNP
Volume
6
Issue
3
Year of publication
2001
Pages
209 - 218
Database
ISI
SICI code
1355-8145(200107)6:3<209:TJPOAT>2.0.ZU;2-U
Abstract
A total of 89 J-domain proteins were identified in the genome of the model flowering plant Arabidopsis thaliana. The deduced amino acid sequences of t he J-domain proteins were analyzed for an assortment of structural features and motifs. Based on the results of sequence comparisons and structure and function predictions, 51 distinct families were identified. The families r anged in size from 1 to 6 members. Subcellular localizations of the A thali ana J-domain proteins were predicted; species were found in both the solubl e and membrane compartments of all cellular organelles. Based on digital No rthern analysis, the J-domain proteins could be separated into groups of lo w, medium, and moderate expression levels. This genomics-based analysis of the A thaliana J-domain proteins establishes a framework for detailed studi es of biological function and specificity. It additionally provides a compr ehensive basis for evolutionary comparisons.