Ja. Miernyk, The J-domain proteins of Arabidopsis thaliana: an unexpectedly large and diverse family of chaperones, CELL STR CH, 6(3), 2001, pp. 209-218
A total of 89 J-domain proteins were identified in the genome of the model
flowering plant Arabidopsis thaliana. The deduced amino acid sequences of t
he J-domain proteins were analyzed for an assortment of structural features
and motifs. Based on the results of sequence comparisons and structure and
function predictions, 51 distinct families were identified. The families r
anged in size from 1 to 6 members. Subcellular localizations of the A thali
ana J-domain proteins were predicted; species were found in both the solubl
e and membrane compartments of all cellular organelles. Based on digital No
rthern analysis, the J-domain proteins could be separated into groups of lo
w, medium, and moderate expression levels. This genomics-based analysis of
the A thaliana J-domain proteins establishes a framework for detailed studi
es of biological function and specificity. It additionally provides a compr
ehensive basis for evolutionary comparisons.