DETERMINATION OF ADENINE AND GUANINE BY CAPILLARY ZONE ELECTROPHORESIS WITH END-COLUMN AMPEROMETRIC DETECTION AT A CARBON-FIBER MICRODISK ARRAY ELECTRODE

Capillary zone electrophoresis was employed to the analysis of adenine and guanine by using end-column amperometric detector with a carbon f iber microdisk array electrode. Optimum conditions of separation and d etection are 0.01 mol/L H3PO4-0.01 mol/L H3BO3-0.01 mol/L HAc-NaOH (pH 9.2) for the buffer solution, 20 kV for the separation voltage, 3.0 k V and 5 s for injection voltage and time, respectively, and 1.00 V (vs .SCE) for the detection potential. The Limit of detection is 8.2 fmol for adenine and 2.9 fmol for guanine (S/N = 2). The method was applied to determination of adenine and guanine in hydrolytic product of yeas t-RNA and calf thymus-DNA.