An efficient microtiter system to determine Agrobacterium biovar

A microtiter system for biovar characterization of Agrobacterium strains wh ich simplifies the analysis of a large number of isolates is described. Thi s method is based on incubation of bacterial strains in microplate wells pr eviously amended with media specifically used by the different Agrobacteriu m biovars. More than 150 purified Agrobacterium strains isolated from the m ost common host plants were analysed by the microtiter system. It proved to be an excellent tool using less reagents, time and space for incubation in comparison with the traditional method.