Partial-filling micellar electrokinetic chromatography and non-aqueous capillary electrophoresis for the analysis of selected agrochemicals

Selected agrochemicals (s-triazines and phenoxy acids) have been investigat ed with partial-filling micellar electrokinetic chromatography (PFMEKC) and non-aqueous capillary electrophoresis (NACE). Because these two techniques are compatible for coupling of capillary electrophoresis with mass spectro metry, different conditions affecting the separation efficiency (reproducib ility, method linearity) were systematically tested, and the results were c ompared with those from classical MEKC. The conditions tested included buff er molarity, pH, the concentrations of the organic modifier and surfactant, the applied voltage, the injection time of the sample, and the length of t he partial-filling plug. The respective limits of detection (LOD) using UV- detection were determined. Reduction of the electrophoretic raw data using the mobility scale transformation (p-scale) improved qualitative comparison of the electropherograms and the reproducibility of quantitative data (int egrated peak area) thus extending this data treatment from CZE to other end oosmotic flow-driven CE-techniques such as PFMEKC and NACE.